Ressource pédagogique : Dr Rodrigo Guabiraba - Precision cut lung slices: a novel versatile tool to examine host–pathogen interactions in the chicken lung

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The avian respiratory tract is a common entry route for many pathogens and an important delivery route for vaccination in the poultry industry. Immune responses in the avian lung have mostly been studied in vivo due to the lack of robust, relevant in vitro and ex vivo models mimicking the mucosal microenvironment. Various principles of microbial infection including pathogen tropism, replication, spread, and innate antimicrobial host responses can readily be studied in suitable cell or tissue culture models. Precision-cut lung slices (PCLS) have the major advantages of maintaining the 3-dimensional architecture of the lung and includes heterogeneous cell populations. PCLS have been obtained from a number of mammalian species and from chicken embryos. However, as the embryonic lung is physiologically undifferentiated and immunologically immature, it is less suitable to examine complex host-pathogen interactions including antimicrobial responses. Together with our partners from the Roslin Institute (Edinburgh, UK), we have recently established the first protocols for preparing and culturing PCLS from immunological mature chickens (chPCLS) and for assessing avian influenza infection and the innate immune response.  Further analyses will be focused on revealing virulence-associated infection and/or host response patterns in chPCLS, which may help to improve our understanding of avian influenza pathogenesis and/or avian influenza virus pathotype evolution in gallinaceous poultry.  

"Domaine(s)" et indice(s) Dewey

• Maladies des poumons (hypertension artérielle pulmonaire, hypertension pulmonaire, maladie obstructive respiratoire chronique, maladies respiratoires, ouvrages généraux sur les maladies des bronches et des poumons) (616.24)

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