Ressource pédagogique : Dr Pawe? Siudem - Aronia melanocarpa Fruits as a Dietary Source of bioactive compounds: 1H?NMR, HPLC-DAD, and Chemometric Studies

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Aronia melanocarpa (Michx.) Elliott's (chokeberry) it is well known for the presence of anthocyanins. The fruits also  contain significant amounts of other bioactive compounds, such as chlorogenic acid and its isomer neochlorogenic acid. They exhibit antioxidant, anti-inflammatory, antidiabetic, and antibacterial activities; thus, they can have a significant impact on the health-promoting properties of chokeberry. In our research, we have determined the changes in the content of chlorogenic acids (CGA) and anthocyanins during the development and ripening of fruits. Aronia fruit samples from two organic farms in Poland, were collected every two weeks from July to October. The methods of NMR spectroscopy and HPLC-DAD were used to determine the chemical composition of the prepared methanolic extracts. The obtained results were analyzed using chemometric analysis and multivariate statistics (PCA). The results showed that the content of chlorogenic acids and anthocyanins changes during ripening and depends on the time of harvest and the region of cultivation. A correlation between the time of CGAs reduction and the appearance of anthocyanins was also noticed. Additionally, the antioxidant properties of extracts were determined using three methods: DPPH, FRAP and ORAC.    

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