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<title><string language="fre"><![CDATA[Prof. Richard Martin - Calcium imaging in the intestine of Ascaris suum, TRP channels and diethylcarbamazine]]></string></title>
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<string language="fre"><![CDATA[The
intestine of nematode parasites is involved in more than digestion, absorption
and storage of nutrients. It is involved in vesicular trafficking, ageing,
innate immunity, drug metabolism and excretion making it a sensitive tissue for
drugs to target. We used Fluo-3 AM, a calcium sensitive dye that is taken up
into the cytoplasm of the columnar cells of isolated Ascaris suum intestine
flaps, to determine the effects of diethylcarbamazine. One of the possible
modes of action of diethylcarbamazine is it is a TRP channel agonist. We used
PCR and detected the presence of TRP channel message in the Ascaris intestine
strips and found: Asu-gon-2, Asu-trp-1(2), Asu-ocr-1, and Asu-trpa-1.
As a control we Increased calcium in the bathing solution to 10mM which
produces a sharp and reversible rise in cytosolic calcium. 10 M
diethylcarbamazine produced a characteristic ‘step-like’ slow increase in
calcium that persisted after removal of diethylcarbamazine before returning
slowly towards base levels. 2-APB, a TRP channel antagonist inhibited the
effect of 10 M
diethylcarbamazine. Interestingly, in the absence of added calcium in the
bathing solution, 10 M
diethylcarbamazine produced a fall in cytosolic calcium as it was leached from
the intestinal cells and 100 M
lanthanum, a TRP channel blocker, prevented this leaching. These observations
suggest a direct effect of diethylcarbamazine on TRP channels of Ascaris intestines,
an effect that is not predicted by effects mediated by host immune systems or
effects on host arachidonic acid metabolism. The observations emphasize the
intestine as a tissue that is sensitive to anthelmintic drugs.
Supported
by NIH R01AI047194
and R01AI155413 to RJM and the EA Benbrook Foundation  ]]></string></description>
<keyword><string language="fre"><![CDATA[ascaris]]></string></keyword>
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<date><dateTime>2021-11-29</dateTime></date>
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